尼古拉·斯拉沃夫

𝐄𝐝𝐮𝐜𝐚𝐭𝐢𝐨𝐧 I received my undergraduate education from the Massachusetts Institute of Technology (MIT) in 2004. Then, I pursued doctoral research in the Botstein laboratory at Princeton University, aiming to understand how cells coordinate their growth, gene expression, and metabolism. We discovered a simple mechanism that can account for the growth-rate dependent transcriptional responses across a wide range of growth conditions and growth rates. Then, I began a postdoctoral project in the van Oudenaarden laboratory at MIT, aiming to understand the Warburg effect, a hallmark of cancer cells characterized by the fermentation of glucose in the presence of enough oxygen to support respiration. This work demonstrated that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival and that, contrary to expectations and decades-long assumptions, exponential growth at a constant rate can represent not a single metabolic/physiological state but a continuum of changing states characterized by different metabolic fluxes. 𝐏𝐫𝐢𝐧𝐜𝐢𝐩𝐚𝐥 𝐢𝐧𝐯𝐞𝐬𝐭𝐢𝐠𝐚𝐭𝐨𝐫 𝐬𝐭𝐚𝐠𝐞 As a fellow at Harvard and the Broad Institute, my research provided direct evidence for differential stoichiometry among core ribosomal proteins in unperturbed wild-type cells. Our findings support the existence of ribosomes with distinct protein composition and physiological function that represent an explored layer of regulating gene expression. In 2015, I started the Slavov laboratory at Northeastern where my team introduced the first methods for Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS and SCoPE2) and used them to quantify proteome heterogeneity during cell differentiation. More recently, we developed second generation methods (pSCoPE, and plexDIA) that substantially increase depth of proteome coverage and data completeness. These technologies provided a foundation for establishing Parallel Squared Technology Institute (PTI) in 2023.